NASC

NASC ID: N16367

Name: SALK_073650 homozygous

ABRC stock number: CS16367

Description: Confirmed line isolated from original SALK_073650 line; homozygous for the insertion; genomic PCR analyses confirmed that a single T-DNA resides within intron 11; sequence analyses of both border fragments located the T-DNA insertion at position 1792 without any base deletion; intron 11 fails to splice due to insertion of a T-DNA copy; lacks N-acetyl-glucosaminyltransferase-I activity and is unable to form complex N-glycans in the Golgi apparatus; fails to react with complex glycan antiserum on immunoblots ("non-stainer" ).

Donation Date: 2008-10-10

Donated by:

Westfälische Wilhelms-Universität Münster Antje Von Schaewen

Donor Number: cgl1-T

Stock type: individual line

Material type: seed

Status	Price (£)
Available	£11.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000, SALK_073650)

Segregation status: homozygous

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
	cgl1-3	cgl1	At4g38240

Phenotype

Longer root hairs compared to wild type. Phenotype curated by ABRC

References

Frank, J. et al. 2008. Comparative analyses of Arabidopsis complex glycan1 mutants and genetic interaction with staurosporin and temperature sensitive3a. Plant physiology 148 (3):1354-67 .PubMed ID: 18768906.